You’ve ever walked into your bathroom and been hit with a sharp, unpleasant urine smell—even after cleaning—you’re not alone. It’s a common issue that many homeowners face, and unfortunately, that stubborn odour doesn’t just vanish with a quick mop or air freshener. Understanding the root causes and using the right techniques can help you get rid of urine smell in bathroom floor once and for all. Let’s dive into the whys and hows of this smelly situation and give your bathroom a breath of fresh air.
Understanding Why Urine Smells Linger on Bathroom Floors
Urine smell tends to linger because it seeps into porous surfaces like tile grout, vinyl seams, and even micro-cracks in your flooring. Once absorbed, the urine dries up, but the uric acid crystals remain. These crystals are reactivated by moisture, making the smell come back again and again—especially in humid environments like bathrooms. Regular cleaning might remove visible messes, but it won’t always reach deep into the material where the real problem hides.
The Most Common Causes of Urine Odours in Bathrooms
Urine odours can build up for several reasons. Accidents during potty training, elderly family members with mobility issues, or even pets mistaking the bathroom for their personal loo are just a few examples. Sometimes, poor aim (we’re looking at you, gents!) or splashes around the toilet base contribute to the problem. Over time, these incidents create a build-up of odour-causing bacteria and urine residue—especially if the floor isn’t sealed properly or cleaned often with the right products.
Why Standard Cleaning Products Might Not Be Enough
Your everyday floor cleaner or multi-surface spray may give your bathroom a nice citrusy scent for a few hours, but that doesn’t mean it’s done the job. Most standard cleaning products are not designed to break down uric acid crystals, which are the true culprits behind that persistent smell. Without enzymes or specialised ingredients, regular cleaners simply mask the odour temporarily, allowing it to return shortly after.
Getting Started: What You’ll Need for Odour Removal Success
To effectively get rid of urine smell in bathroom floor, you’ll need more than just a mop and bucket. Start with an enzyme-based cleaner, which is specifically formulated to break down urine molecules. You might also want gloves, an old toothbrush or grout brush for scrubbing corners, and clean microfibre cloths. If you’re dealing with older stains, a black light can help identify hidden spots. Optional but helpful items include baking soda for deodorising and a steam cleaner for deep sanitisation. Equipped with the right tools, you’ll be ready to tackle the problem from the root—not just the surface.
Step-by-Step: How to Get Rid of Urine Smell in Bathroom Floor Naturally
You’re looking for a safe, eco-friendly way to tackle that stubborn urine smell without using harsh chemicals, you’re in luck. Below is a simple step-by-step guide to get rid of urine smell in bathroom floor naturally using ingredients you probably already have at home.
Step 1: Identify the Source of the Smell

Before diving into cleaning, pinpoint exactly where the smell is coming from. Sniff around the toilet base, behind the toilet, corners of the bathroom, and even around floor drains. For more precision, especially for old stains, you can use a black light to detect urine spots.
Step 2: Prepare a Natural Cleaning Solution

Mix your own odour-fighting solution using:
- 1 cup white vinegar
- 1 cup water
- 2 tablespoons baking soda
- (Optional: 10 drops of essential oil like tea tree or lavender for a pleasant scent and extra antibacterial power)
Pour the mixture into a spray bottle and shake it well.
Step 3: Spray the Affected Area Generously

Liberally spray the solution on the smelly spots, focusing on tile grout lines, corners, and around the base of the toilet. Allow it to sit for 10–15 minutes to break down the urine particles and kill bacteria.
Step 4: Scrub with a Brush

Using a soft scrub brush or an old toothbrush, scrub the area thoroughly. This is especially important if you have tile or textured flooring, where urine can seep into tiny crevices.
Step 5: Wipe and Rinse
Wipe the area with a clean, damp cloth to remove any remaining vinegar and baking soda residue. If needed, rinse again with water and dry with a towel.
Step 6: Use Baking Soda for Extra Deodorising

Once the area is dry, sprinkle a thin layer of baking soda over the spot and let it sit overnight. This helps absorb any lingering odours from deep within the floor. Sweep or vacuum the baking soda in the morning.
Step 7: Keep It Dry and Ventilated

Moisture reactivates urine odours, so make sure the bathroom stays dry and well-ventilated. Use a fan or open a window after showers, and wipe up water spills promptly.
Using Vinegar and Baking Soda to Neutralise Bad Smells
Vinegar and baking soda are a classic duo when it comes to odour control—and for good reason. Vinegar’s natural acidity helps break down urine salts and kill bacteria, while baking soda neutralises lingering smells. Simply spray white vinegar directly onto the affected floor area, let it sit for 10 minutes, then sprinkle baking soda over the top. The fizzing reaction helps lift the odour-causing particles from the surface. After 15–20 minutes, scrub and rinse thoroughly. This natural remedy works best for fresh stains or mild odours.
Hydrogen Peroxide Tricks That Tackle Tough Urine Odours
For more stubborn or set-in urine smells, hydrogen peroxide is your secret weapon. It’s a powerful disinfectant that can penetrate deeper into tile grout or flooring to destroy bacteria and neutralise odours. Mix ½ cup hydrogen peroxide, 1 teaspoon dish soap, and 1 tablespoon baking soda. Apply the mixture to the affected area and scrub it in with a soft brush. Let it sit for 15 minutes before rinsing clean. Be cautious using this method on coloured tiles or natural stone, as peroxide may cause discoloration—always patch test first.
How to Make a DIY Urine-Smell-Fighting Spray That Works
Creating your own urine-odour-fighting spray is both affordable and effective. Combine 1 cup water, ½ cup white vinegar, 1 tablespoon baking soda, and 10 drops of essential oil (such as eucalyptus or tea tree) in a spray bottle. Shake well before each use. Spray liberally on the problem area, let it sit for a few minutes, and then wipe clean with a microfiber cloth. This DIY solution not only targets the odour but leaves your bathroom smelling naturally fresh without harsh chemicals.
Essential Oils That Help Your Bathroom Smell Clean and Fresh
Essential oils don’t just mask unpleasant smells—they also offer antibacterial and antifungal benefits. Tea tree oil is particularly useful for breaking down odours, while lavender, eucalyptus, and lemon oil provide a clean, fresh scent. Add a few drops to your DIY spray or mix with water and a bit of alcohol in a diffuser bottle to keep your bathroom air smelling clean. Bonus: essential oils can help eliminate the stress of that “public restroom” vibe at home.
Dealing with Grout: The Hidden Source of Urine Odour
Grout is often the sneaky culprit when it comes to lingering urine smells. Because it’s porous, it can absorb urine over time, making the smell almost impossible to remove with surface cleaning alone. Start by applying a paste of baking soda and hydrogen peroxide directly onto the grout lines. Scrub with a stiff-bristled brush and let it sit for 10–15 minutes before rinsing clean. For long-term results, consider sealing your grout to prevent future absorption and make cleanup easier.
When the Floor Is the Problem: Porous Tiles and Urine Absorption
You’ve tried everything and that urine smell still lingers, your floor materials might be to blame. Porous tiles, like unsealed stone or old ceramic, can soak up urine like a sponge. Once inside, the odour can be reactivated every time the bathroom gets humid. In these cases, steam cleaning the floor or applying a penetrating enzyme cleaner is your best bet. Long-term, sealing the floor or even replacing heavily damaged tiles may be the only way to fully resolve the issue.
How to Stop the Smell at the Source: Pet and Toddler Toilet Accidents
You have pets or toddlers in the house, accidental bathroom messes are almost inevitable. The key to stopping urine odour at the source is immediate clean-up. Use paper towels to blot up fresh urine, then follow with an enzyme-based cleaner that’s safe for both pets and children. These cleaners break down the proteins and uric acid in urine, eliminating the root of the smell rather than just covering it up. Also, consider using potty-training aids or litter box pads to minimise floor contact and make clean-up quicker and easier.
Preventing Future Urine Smells with Smart Bathroom Habits
Prevention is always better than cure. Encourage all family members (especially young boys!) to sit while using the toilet to reduce splashes. Wipe the base of the toilet regularly, and keep a toilet brush nearby for spot cleaning. Training kids to wipe up accidents immediately and teaching pets to avoid the bathroom altogether can also reduce the chances of repeat offences. Placing a small rug or mat with a waterproof backing near the toilet can help catch accidents and make it easier to keep the area clean.
Floor Mats, Rugs, and Other Items That Might Be Holding Odours
Sometimes it’s not the floor itself—but what’s on it. Bathroom rugs and mats often absorb urine, especially around toilets. If you’ve tried everything and the smell still lingers, try washing your floor mats in hot water with vinegar and baking soda. If the odour persists, it might be time to toss them and invest in machine-washable, quick-drying mats with non-absorbent backing. Avoid heavy fabric rugs that trap moisture and bacteria.
When to Call in the Pros: Deep Cleaning Solutions That Work
You’ve tried all the DIY methods and the smell just won’t go away, it might be time to bring in professional cleaners. Deep cleaning services often use industrial-grade equipment and enzyme treatments that can penetrate deep into tile, grout, and porous materials. They can also identify hidden sources of odour—like subfloor damage or mould buildup. This is especially helpful in older bathrooms where years of small accidents have built up beneath the surface.
Daily and Weekly Cleaning Routines to Keep Urine Smells Away for Good
Establishing consistent cleaning routines is the easiest way to keep odours under control. For daily upkeep, wipe down the toilet base and surrounding floor with a vinegar-water solution or a disinfectant wipe. Once a week, mop the entire floor with an enzyme cleaner or natural odour-neutralising mix, and wash bathroom mats. Don’t forget to scrub grout lines and behind the toilet—often-missed spots where odours can hide. Small efforts add up and can save you from bigger headaches down the line.
Conclusion
Getting rid of urine smell in the bathroom floor doesn’t have to be an endless battle. With the right combination of natural cleaners, smart habits, and regular maintenance, you can banish those unpleasant odours and enjoy a fresher, cleaner bathroom. Whether you’re dealing with toddler accidents, pet mishaps, or old stains, tackling the problem at the source—and keeping up with routine care—makes all the difference. Say goodbye to the stink and hello to a bathroom that truly smells clean!